The influence of diet on faecal DNA amplification and sex identification in brown bears ( Ursus arctos )

The influence of diet on faecal DNA amplification and sex identification in brown bears (Ursus arctos).

To evaluate the influence of diet on faecal DNA amplification, 11 captive brown bears (Ursus arctos) were placed on six restricted diets: grass (Trifolium spp., Haplopappus hirtus and Poa pratensis), alfalfa (Lupinus spp.), carrots (Daucus spp.), white-tailed deer (Odocoileus virginianus), blueberries (Vaccinium spp.) and salmon (Salmo spp.). DNA was extracted from 50 faecal samples of each res...

The influence of diet on faecal DNA amplification and sex identification in brown bears (<i>Ursus arctos</i>)

To evaluate the influence of diet on faecal DNA amplification, 11 captive brown bears ( Ursus arctos ) were placed on six restricted diets: grass ( Trifolium spp., Haplopappus hirtus and Poa pratensis ), alfalfa ( Lupinus spp.), carrots ( Daucus spp.), white-tailed deer ( Odocoileus virginianus ), blueberries ( Vaccinium spp.) and salmon ( Salmo spp.). DNA was extracted from 50 faecal samples o...

Genetic relationships of extant brown bears (Ursus arctos) and polar bears (Ursus maritimus).

Polar bears (Ursus maritimus) and brown bears (Ursus arctos) are closely related species for which extensive mitochondrial and nuclear phylogenetic comparisons have been made. We used previously published genotype data for 8 microsatellite DNA loci from 930 brown bears in 19 populations and 473 polar bears in 16 populations to compare the population genetic relationships of extant populations o...

Management of Brown Bears (Ursus arctos) in Europe

The impact of time and field conditions on brown bear (<i>Ursus arctos</i>) faecal DNA amplification

To establish longevity of faecal DNA samples under varying summer field conditions, we collected 53 faeces from captive brown bears (Ursus arctos) on a restricted vegetation diet. Each faeces was divided, and one half was placed on a warm, dry field site while the other half was placed on a cool, wet field site on Moscow Mountain, Idaho, USA. Temperature, relative humidity, and dew point data w...